As a Master's student with the Wee lab, I am studying the cold stress response of mangroves with the goal of identifying genes involved in the molecular pathway of cold adaptation in mangrove species. To do this I am using a combination of physiological measurements, molecular bench work, and bioinformatics to compare the physiological and molecular response of mangrove species with varying latitudinal limits to cold stress. Our goal is to identify genes involved in the molecular pathway of cold adaptation in mangrove species. These candidate genes for cold tolerance can then be used in future experiments to identify the extent of adaptation to cold stress in natural populations of mangroves. Enabling the prediction of how these populations may respond to changing environmental conditions. In addition, this project will provide a first step in determining the downstream effects of the activation of the cold acclimation pathway in mangroves, and may shed some light on why it is that mangrove species are limited to subtropical and tropical environments.